寡聚核苷酸中鸟苷对四甲基罗丹明的荧光猝灭

具有核苷特异性的荧光猝灭技术在生物领域具有广泛应用.为了更好地理解这一过程的机理及其影响因素,研究了核苷对四甲基罗丹明（TMR）染料的分子间猝灭和在同一条寡聚核苷酸链中的分子内猝灭.与以前的研究结果一致,脱氧单磷酸鸟苷（dGMP）可以有效地猝灭TMR,而其他单磷酸腺苷对其的猝灭可以忽略.由斯特恩-沃尔默图获得TMR和dGMP的双分子猝灭常数为Ks=52.3L/mol.将TMR标记在寡聚核苷酸末端,可以观测到其荧光通过光致电子传递有效地被鸟苷猝灭,我们利用荧光相关光谱的方法测定了这一过程的猝灭速率常数.此外,所得的数据还显示鸟苷附近的碱基会对分子内的猝灭过程产生显著的位阻效应.这些结果将有助于设计寡聚核苷酸荧光探针和理解G猝灭过程.     

基于期权博弈的跨国并购实证分析——以联想并购IBMPC为例

将实物期权定价方法引入到讨价还价谈判博弈中,分别建立了完全信息和不完全信息跨国并购期权博弈模型,并应用纳什讨价还价定理求出了其中不完全信息博弈的唯一解,以及完全信息博弈的解区间.该模型证明期权估值法具有普遍意义, DCF估值法是跨国并购中的一种特例,最后,通过联想并购IBM PC案例检验了模型的基本结论.     

利用高压热处理技术提高Bi-2223/Ag高温超导带材临界电流

提高临界电流密度(Jc)对促进Bi-2223/Ag高温超导带材的应用有着重要意义.在常压热处理条件下所制备的带材中存在大量的裂纹、孔洞和第二相颗粒等,这些因素都会对带材的超导连接性能产生破坏作用.我们成功建立了一套可用氧气氛的热等静压设备,工作气压和温度可同时达到15MPa和850°C.通过引入裂纹愈合热处理过程,我们成功对带材进行了高压热处理,所得样品中第二相颗粒和裂纹数量大幅度降低,77K、自场下的临界电流相比常压热处理样品提高了20%左右.     

Expression and Characterization of an Active Chimeric Protein of Human Extracellular Superoxide Dismutase and hCuZnSOD in Pichiapastoris

Mammalian cells express two isoforms of Cu-and Zn-containing superoxide dismutases(SODs),CuZnSOD and extracellular SOD(EC-SOD),involved in the defense system against reactive oxygen species(ROS).The two SODs have structurally homologous centre domain with distinct N-and C-terminuses,resulting in the different characteristics of the structure and function of the two molecules.We generated a hybrid SOD molecule(namely hySOD) via replacing the N-and C-terminuses of hCuZnSOD with the counterparts of hEC-SOD.The...     

边界振动的微腔中薛定谔猫态的产生

采用全量子理论,考虑了边界振动的微腔(振动边界视作频率为ωm的量子谐振子)与单模辐射场构成的系统,在薛定谔绘景中,给出了系统的时间演化算符,得到了系统的态函数随时间的演化关系.结果表明,当振动边界回到初态时的腔场态能给出许多与已有文献不同的薛定谔猫态,且通过调整单模辐射场的频率可以选择想要的薛定谔猫态.     

DFT Study on Some Properties of Polybrominated Phenoxathiin 10-Oxides

With the B3LYP calculation method of density functional theory(DFT)and the 6-31G* basis set,full optimization calculation was made for phenoxathiin10-oxide(PTO)and 135 polybromine phenoxathiin 10-oxides(PBPTOs)with the Gaussian 03 program and molar heat capacity in constant volume(CVθ)value of each molecule in the standard state was obtained.The relation between CVθ and the substitution position and number of bromine atom(NPBS)was studied,and the results indicated good correlation(R2 = 1.000)between CVθ and NPBS of PBPTO compounds.Based on the output file of Gaussian 03 program,molar heat capacity at constant pressure(Cp,m)of PBPTO compounds from 200 to 1,000 K was calculated with the statistical thermodynamics program,and the correlation equation between Cp,m and temperature(T,T-1 and T-2)was obtained with the least-squares method,and the correlation coefficient of the correlation equation(R2)was 1.000.In addition,based on the partition function of each molecule calculated by vibration analysis,the relative rate constant of formation of each molecule was calculated.     

A Novel Method for Fabrication of a Glass-Electrode-Based Lipase Sensor

Lipase is one of the hydrolases. It catalyzes the hydrolysis of triglycerides, liberating fat acids. Based on its catalysis and the response of a glass electrode to the acids, a glass-electrode-based lipase sensor can be fabricated1, which has great potential in clinical medicine for the detection of blood fat in human serum samples.The immobilization of lipase on the bulb of a glass electrode is the key to the development of the sensor. In addition to its response time, which has a lot to do …     

Microcalorimetric Character of Inhibition of Salicylaldehyde Glucosamine Schiff Base SG on Bacterial Metabolism

ＩｎｔｒｏｄｕｃｔｉｏｎＳｉｎｃｅＳｃｈｉｆｂａｓｅａｎｄｉｔｓｍｅｔａｌｃｏｍｐｌｅｘｅｓａｒｅｏｆａｎｔｉｂａｃｔｅｒｉａｌａｎｄａｎｔｉｃａｎｃｅｒｂｉｏａｃｔｉｖｉｔｉｅｓ，ｔｈｅｙｈａｖｅｂｅｅｎａｒｅｓｅａｒｃｈｓｕｂｊｅｃｔｏｆｍｕｃｈ...     

BZ反应的滴定量热研究

用滴定热量计考察丙二酸(以下简称MA)-溴酸钾-硝酸铈铵-硫酸振荡体系在不同反应物浓度下周期、诱导期、诱导期放热的变化情况. 发现反应物浓度与周期、诱导期的倒数之间存在着线性关系,并观测到溴酸钾与丙二酸的浓度固定在不同的值时,随着Ce4 浓度的逐渐变稀,振荡周期变短,从动力学理论角度对上述现象进行了分析.     

利用EXCEL改革《概率论与数理统计》课堂教学

讨论如何在《概率论与数理统计》课堂上巧用活用EXCEL,提高课堂教学的效率和教学效果.详细介绍了EXCEL教学模板的设计、制作过程与应用方法.